產品櫥窗
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Coomassie Brilliant Blue R-250, powder
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產品型號：101-6104-59-2 商品規格： |
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Protein Experiment, Staining Reagent. Synonym: Acid Blue 83, Brilliant Blue R, Brilliant indocyanin 6B.
C45H44N3NaO7S2
M.W.: 825.97
Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved.
Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates.
Maximum solubilities: 70 mg/ml in water, 20 mg/ml in methyl cellosolve, and 10 mg/ml in ethanol.
Staining solutions at 0.25 % in 40 % methanol plus 7 % acetic acid in deionized water remain suitable for staining PAGE gels stored at room temperature for at least two months.
M.W.: 825.97
Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved.
Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates.
Maximum solubilities: 70 mg/ml in water, 20 mg/ml in methyl cellosolve, and 10 mg/ml in ethanol.
Staining solutions at 0.25 % in 40 % methanol plus 7 % acetic acid in deionized water remain suitable for staining PAGE gels stored at room temperature for at least two months.
Store at Room Temp