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  • Protease Inhibitor Cocktail for His Tag Sequences
  • 產品型號:4016

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Protease inhibitor cocktail is used in purification of Histidine-tagged proteins to increase protein stability.

Protease Inhibitor Cocktail for His Tag Sequences, EDTA-Free

    Crude cell extracts contain numbers of endogenous enzymes, such as proteases and phosphatases, which are capable of quickly degrading the proteins of interest present in the extract. As a result, this biochemical process can drastically reduce the yield of any protein during any isolation step and endanger all further downstream experiments. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material.
    All cells contain a different mixture of enzymes, but the following generalizations can be made: Serine proteases are widely distributed in most type of cells / Bacterial extracts typically contain serine and metalloproteases / Extracts from animal tissues contain mainly serine, cysteine and metalloproteases.
    Some also contain aspartic proteases / Plant extracts contain large amounts of serine and cysteine proteases. Since cells contain different type of enzymes, our specially formulated cocktails of well selected, different inhibitors supplied in a ready-to-use form, will provide complete protection for your proteins of interest for subsequent experiments like Western blot, reporter gene analysis, immunoprecipitations, epitope tagging, specific protein activity assay or during further purification steps.
 
Applications:
WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay etc.
 
Procedure:
Thaw on room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.

Each Set contains the following components:
 Inhibitor  Target Proteases
 AEBSF  Serine Proteases
 Bestatin  Aminopeptidase 
 E-64  Cysteine Proteases
 Pepstatin A   Aspartic Proteases
 Phosphoramidon Disodium Salt metalloproteinase




 
Store at -20℃