產品櫥窗
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Propidium Iodide, >95%
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產品型號：101-25535-16-4 商品規格： |
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Fluorescent stain for nucleic acids and IHC. Synonym: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
C27H34I2N4
Bi-Ying Weia.; Yu-Min Juanga.; Chien-Chen Lai. A novel approach for quantitative peptides analysis by selected electron transfer reaction monitoring. Journal of Chromatography A. 2010, 1217, (44), 6927-6931.
Hsuan-shen Chen.; Tomas Rejtar.; Viktor Andreev.; Eugene Moskoves.; Barry L. Karge. High-Speed, High-Resolution Monolithic Capillary LC-MALDI MS Using an Off-Line Continuous Deposition Interface for Proteomic Analysis. Anal. Chem. 2005, 77, (8), 2323-2331.
PI Counterstain Solution:
PI Stock Solution (1mg/ml or 1.5 mM):
To prepare, add 1 mg PI (Propidium Iodide) to 1 ml distilled water. Store stock solution at 4ºC (or aliquot and store at -20ºC), protecting from light. When handled properly, solutions are stable for many years.
RNase A Stock Solution (1mg/ml):
To prepare, add 1 mg RNase A to 1 ml distilled water. Aliquot and store at -20ºC freezer.
PI Working Solution (1 ug/ml PI and 10 ug/ml RNase A in PBS):
To prepare, add 2 ul PI Stock Solution and 20 ul RNase A Stock Solution to 2 ml PBS
Incubate sections in dark for 30 minutes at room temperature or 20 minutes at 37 ºC.
Staining Pattern
Nuclei will be stained red.
Suggested Use
Counterstaining for immunofluorescence when green (FITC) or blue (AMCA) fluorescent marker is used.
M.W.: 668.39
Propidium iodide is a fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.Bi-Ying Weia.; Yu-Min Juanga.; Chien-Chen Lai. A novel approach for quantitative peptides analysis by selected electron transfer reaction monitoring. Journal of Chromatography A. 2010, 1217, (44), 6927-6931.
Hsuan-shen Chen.; Tomas Rejtar.; Viktor Andreev.; Eugene Moskoves.; Barry L. Karge. High-Speed, High-Resolution Monolithic Capillary LC-MALDI MS Using an Off-Line Continuous Deposition Interface for Proteomic Analysis. Anal. Chem. 2005, 77, (8), 2323-2331.
PI Counterstain Solution:
PI Stock Solution (1mg/ml or 1.5 mM):
To prepare, add 1 mg PI (Propidium Iodide) to 1 ml distilled water. Store stock solution at 4ºC (or aliquot and store at -20ºC), protecting from light. When handled properly, solutions are stable for many years.
RNase A Stock Solution (1mg/ml):
To prepare, add 1 mg RNase A to 1 ml distilled water. Aliquot and store at -20ºC freezer.
PI Working Solution (1 ug/ml PI and 10 ug/ml RNase A in PBS):
To prepare, add 2 ul PI Stock Solution and 20 ul RNase A Stock Solution to 2 ml PBS
Incubate sections in dark for 30 minutes at room temperature or 20 minutes at 37 ºC.
Staining Pattern
Nuclei will be stained red.
Suggested Use
Counterstaining for immunofluorescence when green (FITC) or blue (AMCA) fluorescent marker is used.
Store at 4℃
Hygroscopic. Light Sensitive.
Hygroscopic. Light Sensitive.