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  • 3,3′,5,5′-Tetramethylbenzidine, TMB, >99%
  • 產品型號:101-54827-17-7

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3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. Synonym: BM blue, Sure Blue TMB, TMB substrate, TMB

[(-C6H2(CH3)2-4-NH2]2
M.W.: 240.34

    Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.


Tang, S.; Chang, Y.; Chia, G. H.; Lee, H. K. Selective extraction and release using (EDTA-Ni)-layered double hydroxide coupled with catalytic oxidation of 3,3',5,5'-tetramethylbenzidine for sensitive detection of copper ion. Anal. Chim. Acta 2015, 885, 106-113.
Guo, Y.; Ma, Q.; Cao, F.; Zhao, Q.; Ji, X. Colorimetric detection of hypochlorite in tap water based on the oxidation of 3,3',5,5'-tetramethylbenzidine. Anal. Methods 2015, 7 (10), 4055-4058.
Strore at 4℃.
Keep Dry. Protect from light.