產品櫥窗
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Trypan Blue
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產品型號：101-72-57-1 商品規格： |
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Trypan blue is a dye used to distinguish between live and dead cells.
C34H24N6Na4O14S4
M.W.: 960.81
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.
For 0.4% Stock Solution preparation:
If dye content is 40%, weight 1g of dye to 100mL ddH2O or 1x PBS.
If dye content is 60%, weight 0.667g of dye to 100mL ddH2O or 1x PBS.
McMahon TA.; Rohr JR. Trypan blue dye is an effective and inexpensive way to determine the viability of Batrachochytrium dendrobatidis zoospores.EcoHealth2014,11(2), 164-167.
Serafini R.; Longobardi V.; Spadetta M.; Neri D.; Ariota B.; Gasparrini B.; Di Palo R. Trypan blue/giemsa staining to assess sperm membrane integrity in salernitano stallions and its relationship to pregnancy rates.Reproduction in Domestic Animals = Zuchthygiene,2014,49(1), 41-47.
M.W.: 960.81
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell cluster cell viability from caprine pancreatic samples. It is also useful to observe hyphea of fungi and Stramenopiles. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.
For 0.4% Stock Solution preparation:
If dye content is 40%, weight 1g of dye to 100mL ddH2O or 1x PBS.
If dye content is 60%, weight 0.667g of dye to 100mL ddH2O or 1x PBS.
McMahon TA.; Rohr JR. Trypan blue dye is an effective and inexpensive way to determine the viability of Batrachochytrium dendrobatidis zoospores.EcoHealth2014,11(2), 164-167.
Serafini R.; Longobardi V.; Spadetta M.; Neri D.; Ariota B.; Gasparrini B.; Di Palo R. Trypan blue/giemsa staining to assess sperm membrane integrity in salernitano stallions and its relationship to pregnancy rates.Reproduction in Domestic Animals = Zuchthygiene,2014,49(1), 41-47.
Store at Room Temperature.
Caution: Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
Caution: Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.