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  • ATP, 100mM Solution
  • 產品型號:102-987-65-5

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Aqueous solution for RNA use. Synonym: Adenosine-5'-Triphosphate.

C10H13N5O13P3
M.W.: 507.18
99% Min.

For use in DNA-dependent RNA polymerase transcription

Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo. ATP is use in many cellular processes, respiration, biosynthetic reactions, motility, and cell division. ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase(s) that produce the second messenger cAMP. ATP provides the metabolic energy to drive metabolic pumps. ATP serves as a coenzyme in a wide array of enzymatic reactions.

This Molecular Biology Reagent is a 100 mM aqueous solution of ATP with the pH adjusted to approximately 7.0 with Tris base. No DNase or RNase has been detected in the solution. This reagent is for use in molecular biology applications such as kinase reactions or DNA dependent RNA polymerase transcription.

ATP is soluble in water (50 mg/ml), yielding a clear, colorless solution. This solution is mildly acidic (pH approximately 3.5).

Stock Conc.: 100mM in water, Store at -20°C
Working Conc.: 5mM ATP(Measurement of luciferase activity in extracts from transiently transfected cells.  pH  must be adjusted.)
Store at -20℃.