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  • IPTG (Isopropyl-β-D-thiogalactoside)
  • 產品型號:101-367-93-1

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Inducer ​for microbiology culture. Synonym: Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside.

C9H18O5S
M.W.: 238.3
Assay (HPLC): >98% 
Dioxane (GLC): Not detected

    The most commonly used synthetic inductor of the Lac operon is IPTG since it is both active at very low concentrations and not subject to enzymatic degradation.

Stock Conc.: Dissolve 100mM or 500mM IPTG in water or buffer, sterilize by filtration through a 0.22um filter and store at -20°C.
Working Conc.: 0.1-1mM

Yansura, D.G. & Henner, D.J. (1984) Proc. Natl. Acad. Sci. USA 81, 439-443
Use of the E.coli lac repressor and operator to control gene expression in B. subtilis.
Cho, S. et al. (1985) Biochem. Biophys. Res. Com. 128, 1268-1273
Effect of IPTG concentration on the level of Lac operon induction in steady state E. coli.

 
Store at -20℃.
Protect from light.