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  • Glycine
  • 產品型號:101-56-40-6

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For use in electrode buffers for PAGE and in transfer buffers for Western blots. Synonym: Aminoacetic acid, Aminoethanoic acid, Glycocoll

NH2CH2COOH
M.W.: 75.07
White Crystalline Powder

    Glycine is a component of Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.
    The most commonly used buffer in the polyacrylamide gel electrophoresis for proteins is based on the work of Laemmli. The electrode buffer (pH 8.3) consists of 0.1% SDS, 0.025M Tris and 0.192M Glycine.

Laemmli, U.K. (1970) Nature 227, 680-685
Cleavage of structural proteins during the assembly of the head of Bacteriophage T4.


 
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