產品櫥窗
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Total RNA Isolation Kit-Tissue
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產品型號：TRNAT 商品規格： |
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Column-based purification of high quality RNA from fresh, frozen tissue or Paraffin-embedded tissue.
Tissue Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from tissue sample. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffer system allows the bases of RNA species to bind to the the glass fiber matrix of the spin column. Contaminants pass through the column easily and impurities are efficiently washed away. Purified RNA is eluted with REL Buffer without requirement of phenol extraction or alcohol precipitation.
RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.
RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.
Sample: Up to 30mg of tissue or 25mg of paraffin-embedded tissue
Yield: Up to 30ug Total RNA
Comparison data with I Brand:
| Conc. (μg/ml) |
OD 260/280 | OD 260/230 | |
| I Brand | 16.5 | 1.44 | 0.21 |
| I Brand | 17.8 | 1.46 | 0.17 |
| Cyrus | 56.6 | 2.06 | 1.71 |
| Cyrus | 58.3 | 2.01 | 1.68 |
Store at Room Temperature.