產品櫥窗
-
NADH disodium salt, >95%
|
產品型號：101-606-68-8 商品規格： |
 |
β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate. Synonym: β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form
C21H27N7Na2O14P2
M.W.: 709.40
Assay.: 95% min.
Reduced form Disodium Salt
NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Composition of the buffer for the reduction of oxaloacetate:
30 mM potassium phosphate pH 7.5, 0.5 mM oxaloacetate, 0.1 mM NADH, 20 mM KCl, 5 mM MgCl2.
Composition of the buffer for the reduction of pyruvate:
100 mM MOPS pH 6.1, 10 mM Na-pyruvate, 100 mM Na2CO3, 0.1 mM NADH, 5 mM MgCl2.
Kobayashi, K. et al. (1989) J. Biol. Chem. 264, 3200-3205
Structure and properties of malic enzyme from Bacillus stearothermophilus.
M.W.: 709.40
Assay.: 95% min.
Reduced form Disodium Salt
NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Composition of the buffer for the reduction of oxaloacetate:
30 mM potassium phosphate pH 7.5, 0.5 mM oxaloacetate, 0.1 mM NADH, 20 mM KCl, 5 mM MgCl2.
Composition of the buffer for the reduction of pyruvate:
100 mM MOPS pH 6.1, 10 mM Na-pyruvate, 100 mM Na2CO3, 0.1 mM NADH, 5 mM MgCl2.
Kobayashi, K. et al. (1989) J. Biol. Chem. 264, 3200-3205
Structure and properties of malic enzyme from Bacillus stearothermophilus.
Store at -20℃
Shipping at Room Temp within Taiwan; may vary elsewhere
Shipping at Room Temp within Taiwan; may vary elsewhere