產品櫥窗
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LB Broth
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產品型號：21100 商品規格： |
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For Microbiology Culture. Less Dust. Synonym：Luria-Bertani Broth (Miller)
Miller's LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.
Stir to suspend 12.5g powder in 500mL water. Autoclave at 121°C for 15 minutes to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Components (25g/ 1L H2O):
Tryptone: 10g
Yeast Extract: 5g
NaCl: 10g
Stir to suspend 12.5g powder in 500mL water. Autoclave at 121°C for 15 minutes to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Components (25g/ 1L H2O):
Tryptone: 10g
Yeast Extract: 5g
NaCl: 10g
Store at Room Temperature.