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Protease Inhibitor Cocktail
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產品型號：4013 商品規格： |
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A cocktail of six protease inhibitors for mammalian cell and tissue extracts. 0.5M EDTA in addition. For bacterial cell proteins extraction, EDTA is suggested.
Appearance:
A: Liquid in 1ml DMSO
B: Liquid in 1ml ddH2O
Procedure:
Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications:
WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
A: Liquid in 1ml DMSO
B: Liquid in 1ml ddH2O
Procedure:
Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications:
WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
Each Set contains the following components:
Note:
EDTA inhibits metalloproteases by chelating the divalent cations that is necessary for their activity, so the activities of other proteins may be affected by EDTA. Therefore, empirical testing may be need in particular experiments to determine if EDTA will take a bad impact. If the protein of interest is to be purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed by 2D gel electrophoresis, you can use solution A only.
| Inhibitor | Concentration | Target Proteases | |
| A(100X in DMSO) | AEBSF | 104 mM | Serine Proteases |
| Aprotinin | 80 μM | Broad Spectrum, Serine Proteases | |
| Bestatin | 5 mM | Aminopeptidase B and Leucine Aminopeptidase | |
| E-64 | 1.5 mM | Cysteine Proteases | |
| Leupeptin | 2 mM | Cysteine Proteases and Trypsin-like Proteases | |
| Pepstatin A | 1.5 mM | Aspartic Proteases | |
| B(100X in ddH2O) | EDTA | 0.5M | Protease |
EDTA inhibits metalloproteases by chelating the divalent cations that is necessary for their activity, so the activities of other proteins may be affected by EDTA. Therefore, empirical testing may be need in particular experiments to determine if EDTA will take a bad impact. If the protein of interest is to be purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed by 2D gel electrophoresis, you can use solution A only.