產品櫥窗
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5-Fluoroorotic Acid (5-FOA)
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產品型號：101-703-95-7 商品規格： |
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Yeast selection reagent. Synonym: 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid, 5-Fluorouracil-4-carboxylic acid
C5H3FN2O4
M.W.: 174.09
Typical Purity (HPLC): >98%
Orotic Acid (HPLC): <2%
5-Fluorouracil: None detected
Melting point: 256-259℃
Solubility: 50mg/mL (4M NH2OH);
Solubility in dimethylsulfoxide(DMSO) is greater than 150 mg/mL
Applications
•Detection of URA3 gene expression in yeast
•Construction of yeast 2-hybrid libraries
•Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations
5-Fluoroorotic acid is a pyrimidine analogue and used for the selection of Saccharomyces cerevisiae with mutations in the URA3-locus (ura3--cells) and Schizosaccharomyces pombe. Wilde type strains of yeast or mutants with a plasmid carrying the URA3-sequence cannot grow in the presence of 5-FOA. Mutations in the URA3-gene result in the loss of activity of the Orotidin-5'-phosphate decarboxylase.
Working Conc.: 1mg/mL - 500ug/mL (500ug/mL will get higher background)
Normally a minimal medium for 5-FOA-selection is used, since in rich medium (YPD) different URA3+-strains may grow in the presence of 5-FOA.
M.W.: 174.09
Typical Purity (HPLC): >98%
Orotic Acid (HPLC): <2%
5-Fluorouracil: None detected
Melting point: 256-259℃
Solubility: 50mg/mL (4M NH2OH);
Solubility in dimethylsulfoxide(DMSO) is greater than 150 mg/mL
Applications
•Detection of URA3 gene expression in yeast
•Construction of yeast 2-hybrid libraries
•Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations
5-Fluoroorotic acid is a pyrimidine analogue and used for the selection of Saccharomyces cerevisiae with mutations in the URA3-locus (ura3--cells) and Schizosaccharomyces pombe. Wilde type strains of yeast or mutants with a plasmid carrying the URA3-sequence cannot grow in the presence of 5-FOA. Mutations in the URA3-gene result in the loss of activity of the Orotidin-5'-phosphate decarboxylase.
Working Conc.: 1mg/mL - 500ug/mL (500ug/mL will get higher background)
Normally a minimal medium for 5-FOA-selection is used, since in rich medium (YPD) different URA3+-strains may grow in the presence of 5-FOA.
Store at -20℃
Harmful!
Harmful!