產品櫥窗
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TG Buffer 10X
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產品型號：200A001 商品規格： |
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A transfer buffer for Western blotting. Synonym：Tris-Glycine Buffer 10X
For Western blotting and gel electrophoresis, Tris-Glycine buffer is used to make a Tris-Glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers.
The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. The 10x TG buffer is diluted to 1x with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol.
A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
10X TG stock solution (1 Liter) contains:
30 g of Tris base
144 g of Glycine
Ready to use. Before use, add 9X of ddH2O to dilute.
The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. The 10x TG buffer is diluted to 1x with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol.
A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
10X TG stock solution (1 Liter) contains:
30 g of Tris base
144 g of Glycine
Ready to use. Before use, add 9X of ddH2O to dilute.
Store at Room Temperature.