Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH. The osmolarity and ion concentrations of the solutions match those of the human body (isotonic).

Application：
PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing. PBS with EDTA is also used to disengage attached and clumped cells. Divalent metals such as zinc, however, cannot be added as this will result in precipitation. For these types of applications, Good's buffers are recommended.

10X PBS stock solution (1 Liter) contains:
80g of NaCl
2g of KCl
14.4g of Na2HPO4
2.4g of KH2PO4
Ready to use. Before use, add 9X of ddH₂O to dilute.  

※pH turns to 7.4 after diluted to 1X.  (pH of 10X stock is 6.8)
※Low-temperature may cause precipitation. If so, heat it in water bath until dissolved.



 

 

 

Stored at RT.
4ºC for longer time.