產品櫥窗
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Potassium Phosphate Dibasic, Anhydrous(K2HPO4)
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產品型號：101-7758-11-4 商品規格： |
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Potassium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Synonym: Dipotassium phosphate anhydrous, Dipotassium phosphate
K2HPO4
M.W.: 174.18
Physical State: Solid
Appearance: White powder
Odour: Odourless
Assay: 99.0%
Solubility : 100 mg/mL in water, yielding a clear, colorless solution
Potassium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Most neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Some limitations of the usefulness of phosphate buffers include their precipitation of Ca2+ and Mg2+, their inhibition of restriction enzyme activity, and their interference in protocols related to DNA ligation and bacterial transformation.
1. Use of high concentrations of potassium phosphate in the immobilization of affinity ligands onto epoxide-activated stationary phases.
2. Use a two-phase system of aqueous potassium phosphate and poly (ethylene glycol) for the isolation of E. coli β-galactosidase and β-galactosidase fusion proteins.
3. Use the strong cation and anion exchange HPLC guard columns and potassium phosphate solution for the quantitation of nonionic surfactants in buffered solutions.
M.W.: 174.18
Physical State: Solid
Appearance: White powder
Odour: Odourless
Assay: 99.0%
Solubility : 100 mg/mL in water, yielding a clear, colorless solution
Potassium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Most neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Some limitations of the usefulness of phosphate buffers include their precipitation of Ca2+ and Mg2+, their inhibition of restriction enzyme activity, and their interference in protocols related to DNA ligation and bacterial transformation.
1. Use of high concentrations of potassium phosphate in the immobilization of affinity ligands onto epoxide-activated stationary phases.
2. Use a two-phase system of aqueous potassium phosphate and poly (ethylene glycol) for the isolation of E. coli β-galactosidase and β-galactosidase fusion proteins.
3. Use the strong cation and anion exchange HPLC guard columns and potassium phosphate solution for the quantitation of nonionic surfactants in buffered solutions.
Store at Room Temperature.