產品櫥窗
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HEPES
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產品型號：101-7365-45-9 商品規格： |
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Molecular Biology Buffer. Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
C8H18N2O4S
M.W.: 238.3０
HEPES is a widely used buffer in biological studies. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10-15mM).
The addition of 20mM HEPES to TBE buffer improves the migration behavior of certain samples in the SSCP analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the sequences between certain bands are wanted.
Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-310 Evaluation of HEPES as a tissue culture buffer.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.
M.W.: 238.3０
HEPES is a widely used buffer in biological studies. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10-15mM).
The addition of 20mM HEPES to TBE buffer improves the migration behavior of certain samples in the SSCP analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the sequences between certain bands are wanted.
Good, N.E. et al. (1966) Biochemistry 5, 467-477 Hydrogen ion buffers for biological research.
Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-310 Evaluation of HEPES as a tissue culture buffer.
Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310 Hydrogen ion buffers for biological research.
Store at Room Temp.