產品櫥窗
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X-GlcA
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產品型號：101-129541-41-9 商品規格： |
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Enzymatic Colorimetric Reagent, for GUS Detection. Synonym: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, BC-Indicator, X-glucuronide
C14H12BrClNO7Na
M.W.: 444.6
Assay: 98% min.
Solubility: 2% in DMSO, Clear and Colorless.
X-GlcA, 5-Bromo-4-chloro-3-indolyl-b-D-Glucuronic acid is a substrate for b-D-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative histochemical marker of specific GUS expression in cells and tissue. XglcA is cleaved by GUS at the b1 glucuronic bond between glucuronic acid and the 5-Bromo-4-chloro-3-indolyl part of X-glcA via hydrolysis. The cleavage of X-glcA results in the precipitation of water insoluble blue dichloro-dibromo-indigo precipitate at the site of enzymatic cleavage. Colour formation requires three separate reactions.After enzymatic turnover, the released indoxyl derivative dimerizes and is subsequently oxidized to the final indigo dye.
Note:
X-GlcA, sodium salt is unstable in water. Use immediately after dissolution. X-GlcA solution should be added to media after it is cooled below 55 °C.
M.W.: 444.6
Assay: 98% min.
Solubility: 2% in DMSO, Clear and Colorless.
X-GlcA, 5-Bromo-4-chloro-3-indolyl-b-D-Glucuronic acid is a substrate for b-D-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative histochemical marker of specific GUS expression in cells and tissue. XglcA is cleaved by GUS at the b1 glucuronic bond between glucuronic acid and the 5-Bromo-4-chloro-3-indolyl part of X-glcA via hydrolysis. The cleavage of X-glcA results in the precipitation of water insoluble blue dichloro-dibromo-indigo precipitate at the site of enzymatic cleavage. Colour formation requires three separate reactions.After enzymatic turnover, the released indoxyl derivative dimerizes and is subsequently oxidized to the final indigo dye.
Note:
X-GlcA, sodium salt is unstable in water. Use immediately after dissolution. X-GlcA solution should be added to media after it is cooled below 55 °C.
Store at 4℃. Protect from light and humidity.
For long term storage hold at -20℃.
For long term storage hold at -20℃.