C22H29N7O5 · 2HCl
M.W.: 544.43
Purity (TLC) : >99%

Antibiotic used for selecting mammalian cell lines ,which have been transformed by vectors that express puromycin-N-acetyl-transferase. 

Puromycin is a aminonucleoside antibiotic from Streptomyces alboniger. It is an analogon to aminoacyl-tRNA and inhibits the protein synthesis by termination of the peptidyl transfer at the ribosomes in prokaryotes and eukaryotes. The antibiotic inhibits the growth of gram positive bacteria and different animal cells. Fungi and gram negative bacterias are resistant, since puromycin cannot pass the cell wall. The discovery of the puromycin-N-acetyltransferase (PAC) in a Streptomyces-stem allowed to employ the antibiotic as a selection marker, comparable to neomycin.
 

Soluble in water (50 mg/mL), ethanol (~1 mg/mL), DMSO (~13 mg/mL), DMF (~14 mg/mL), and PBS, pH 72 (~10 mg/mL)

Stock conc.: 5-50 mg/mL in dH2O, store at -20℃

Working conc.: 1-30 μg/mL (mammalian cell)

When preparing the stock solution, add appripraite puromycin powder in ddH2O, stir over 2 hours and pass through 0.22um filter to get a strilized solution.

Sambrook, J., Fritsch, E.F. & Maniatis, T.Molecular Cloning: A Laboratory Manual, 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 
 

Store at -20℃

Warning! Toxic!
May be Carcinogenic. Wear gloves and mask when handling product. Avoid contact by all modes of exposure.

Toxic