Appearance: Free-flowing powder
Moisture: <15% 
Gel Strength: >850 g/cm²
pH: 5.0 – 8.0
Transparency: >78 %
Coliform: <30 MPN/100g
Mold and Yeast: <400 CFU/g
*Plant Tissue Culture Tested 

Gellan gum is an agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Application
Gellan Gum is intended for use in place of agar and other gelling agent for plant tissue culture. Gellan Gum has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Gellan Gum has been used:
• in the solidification of culture medium for root assays in Arabidopsis thaliana
• as a component of Solid Paul′s medium for Agrobacterium culture
• as a component of root induction medium in Ilex dumosa tissue culture

Gellan gum is typically used at 2 to 4 g/L. It is suspended in medium that is room temperature or colder. Attempting to suspend it in hot medium will usually result in an incomplete, lumpy suspension that will not melt and dissolve uniformly when autoclaved. It should be added to medium after all heat-stable supplements have been added.

CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants Delay C, et al. Journal of Experimental Botany 64(17), 5383-5394, (2013)
Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells Buschmann H, et al. The New phytologist 190(1), 258-267, (2011)
Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system Luna C, et al. Biocell 27(2), 205-212, (2003)