產品櫥窗
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Nitroblue tetrazolium chloride, NBT, >98%
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產品型號：101-298-83-9 商品規格： |
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Enzymatic Colorimetric Reagent for Alkaline phosphotase. Synonym: Nitrotetrazolium Blue Chloride, p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, Nitro BT
C40H30N10O6 · 2Cl
M.W.: 817.64
Appearance: Yellow Crystalline Powder
Assay: >98%
Loss on Drying: <5%
The most popular application of NBT is the determination of the activity of alkaline phosphatase in combination with BCIP. This reaction is based on the oxidation of indoxyl to the insoluble, blue indigo by NBT. A particularly sensitive and clean material to give blue-violet formazan at 530nm.
NBT Stock conc.: 50 mg/mL in 70% dimethylformamide, store in dark
X-Phos (BCIP) Stock conc.: 50 mg/mL in 100% dimethylformamide, store in dark
M.W.: 817.64
Appearance: Yellow Crystalline Powder
Assay: >98%
Loss on Drying: <5%
The most popular application of NBT is the determination of the activity of alkaline phosphatase in combination with BCIP. This reaction is based on the oxidation of indoxyl to the insoluble, blue indigo by NBT. A particularly sensitive and clean material to give blue-violet formazan at 530nm.
NBT Stock conc.: 50 mg/mL in 70% dimethylformamide, store in dark
X-Phos (BCIP) Stock conc.: 50 mg/mL in 100% dimethylformamide, store in dark
Prepare the BCIP/NBT developing solution just before use as follows:
1. Add 33 μL of the NBT solution to 5 mL of AP buffer and mix well.
2. Add 16.5 μL of the stock solution of BCIP. Mix again. Protect the solution from strong light and use it within 1 hour.
Store at 4℃.
Protect form light!
Protect form light!