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  • TCBS Agar
  • 產品型號:TCBSA

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Vibrio Selective Agar, Thiosulfate Citrate Bile Sucrose Agar

Description:
Thiosulfate citrate bile salts sucrose (TCBS) Agar uses an alkaline pH (8.6), ox bile and 1% NaCl to prevent the growth of gram positive bacteria, members of the Enterobacteriaceae family and the genus Pseudomonas.

Applications:
Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species.

Preparation method:
Weigh 89 grams of this product, add to 1000ml of distilled water, dissolve by boiling, sterilize, and pour into the sterilization plate when cold to 50-55℃. Spare.

Composition: g/L
Polyvalent peptone: 10
Sodium cholate: 3
Sodium citrate: 10
Bovine bile powder: 5
Bromothymol blue: 0.04
Agar: 15
Sodium chloride: 10
Ferric citrate: 1
Yeast extract powder: 5
Sodium thiosulfate: 10
Thymol blue: 0.04
Sucrose: 20
pH: 8.6±0.2
Store at 4°C.