m-HPC Agar was developed by Taylor and Geldreich in 1979 in their pursuit of a suitable standard methods medium to use with the membrane filter procedure. m- HPC Agar is also known as m-Heterotrophic Plate Count Agar and previously as membrane filter Standard Plate Count Agar, m-SPC Agar. The formulation was evaluated by many investigators who reported it as a suitable alternative medium for standard plate counts. It is recommended for the membrane filter method in the recent editions of Standard Methods for the Examination of Water and Wastewater.
    The advantages of the membrane filter procedure over the standard plate count method have been described by many investigators. The volume of inoculum is limited with both pour and spread plate techniques while the membrane filter method enables the use of large samples, which is desirable for water with low counts.

Principles of the Procedure:
    Peptone provides nitrogen and carbon as well as other nutrients. The original concentration of 5% gelatin was reduced to 2.5% to avoid problems associated with liquefying gelatin and spreading colonies.

Directions:
    Dissolve 60g in 1 liter of distilled water and add 10ml glycerol. Mix well and distribute into final containers. Sterilize by autoclaving at 121ºC for 5 minutes.


Composition：(In 60g m-HPC Agar/1L H2O)
Peptone : 20g
Gelatin Peptone : 25g
Agar : 15g
Glycerol : 10mL(additionally add before autoclave)

Store at Room Temp.