Media with Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate are included in many applications for the study of yeasts in molecular genetics. Yeasts are unicellular, eukaryotic, budding cells that are generally round-to-oval or elongate in shape. They multiply principally by the production of blastoconidia (buds). Yeast colonies are moist and creamy or glabrous to membranous in texture. Yeasts are considered opportunistic pathogens.

Features:
‧ Contains all essential nutrients and vitamins necessary for the cultivation of yeasts and a source of nitrogen and carbohydrate
‧ Lacks the amino acids histidine, methionine and tryptophan and ammonium sulfate
‧ Prepared according to Guenter’s modification of Wickerham’s Yeast Nitrogen Base formulation
‧ Displays a good cultural response to Kloeckera apiculata 9774 and Saccharomyces cerevisiae 9080

Components:
Vitamins:
Biotin 2μg/L
Calcium pantothenate 400μg/L
Folic acid 2μg/L
Inositol 2000μg/L
Niacin 400μg/L
p-Aminobenzoic Acid 200μg/L
Pyridoxine hydrochloride 400μg/L
Riboflavin 200μg/L
Thiamine hydrochloride 400μg/L
Trace Elements:
Boric acid 500.0μg/L
Copper sulfate 40.0μg/L
Potassium iodide 100.0μg/L
Ferric chloride 200.0μg/L
Manganese sulfate 400.0μg/L
Sodium molybdate 200.0μg/L
Zinc sulfate 400.0μg/L
Minerals:
Monopotassium phosphate 1.0g/L
Magnesium sulfate 0.5g/L
Sodium chloride 0.1g/L
Calcium chloride 0.1g/L

Note: Suspend 1.9 gram powdered medium in 1 litre distilled water

Store at RT. Keep cool and dry. 
For long-term storage, store at 4℃