YPD broth is used for the propagation and maintenance of yeasts.

General methods in yeast genetics specify using yeast extract-peptone-dextrose (YPD) medium for cultivating Saccharomyces cerevisiae and other yeasts. Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes.

● Yeast extract supplies B-complex vitamins, which stimulate bacterial growth
● Peptone acts as a source of carbon, nitrogen, vitamins and minerals
● Glucose is the carbohydrate source

Components
Formula                            (g/L)
Yeast extract                       10
Bacteriological peptone     20
Glucose                               20
Agar                                    20

Suspend 70 gram powdered medium in 1 litre distilled water
 

Store at Room Temp.