RIPA(Radioimmunoprecipitation Assay) lysis buffer  is used to lyse cells and tissues for use in  radioimmunoprecipitation, protein assays, protein purification and other analytical procedures. Due to its ionic detergent composition, RIPA can disrupt nuclear membranes and solubilize cytoplasmic proteins, resulting in a high protein yield. 

Composition: 
50mM Tris-HCl, 150mM Sodium chloride, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS

Note: RIPA Lysis Buffer does not contain any protease inhibitors or phosphatase inhibitors.  If desired, add inhibitors to RIPA Lysis Buffer immediately before applying to cells.