產品櫥窗
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DEPC
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產品型號：101-1609-47-8 商品規格： |
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for RNA experiment, RNase deactivator. Synonym: Diethyl pyrocarbonate, DEP, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
O(COOC2H5)2
M.W.: 162.14
Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.
Application
Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
For the treatment of water with DEPC, add 1 ml of DEPC to 1 liter bi-distilled water (0.1 % DEPC v/v) and stir over night. Autoclaving 20 minutes inactivates DEPC. DEPC reacts with water and hydrolyses to ethanol and CO2.
M.W.: 162.14
Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.
Application
Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
For the treatment of water with DEPC, add 1 ml of DEPC to 1 liter bi-distilled water (0.1 % DEPC v/v) and stir over night. Autoclaving 20 minutes inactivates DEPC. DEPC reacts with water and hydrolyses to ethanol and CO2.
Store at 4ºC
Caution: DEPC is suspected to be a carcinogen and should be handled with great care. Do not breathe fumes and avoid contact with skin! DEPC may damage eyes and mucous membranes!
Harmful, Irritant
!
Caution: DEPC is suspected to be a carcinogen and should be handled with great care. Do not breathe fumes and avoid contact with skin! DEPC may damage eyes and mucous membranes!
Harmful, Irritant
!