產品櫥窗
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CHAPS
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產品型號：101-75621-03-3 商品規格： |
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3-[(3-Cholamidopropyl)-dimethylammonio]-propane-sulfonate. Molecular Biology Buffer, A Non-denaturing Zwitterionic Detergent for Membrane Biochemistry.
C32H58N2O7S
M.W.: 614.88
Assay (from N): 99.94%
TLC: one spot
Loss on Drying: 0.96%
Solubility (10% in water, 25ºC): Clear and colorless
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent for solubilizing membrane proteins and used for protein solubilization in isoelectric focusing and
especially for non- denaturing (without urea) IEF. CHAPS has been shown to give excellent resolution of some subcellular breaking protein-protein interactions.
Commonly two-dimensional electrophoresis, preparations and plant proteins.
Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT),
Furthermore, its small micellar molecular weight (6,150) and high critical micellar
concentration (6 - 10 mM) allow it to be removed from samples by dialysis.
CHAPS allows the isolation of many proteins in their native state, without loosing much of its activity. Protein-protein interactions can be interrupted more efficiently as with Triton X-100 or sodium cholate.
CHAPS interferes with the Lowry protein assay.
Working Conc.: 1-13mM
Hjelmeland, L.M. (1980) Proc. Natl. Acad. Sci. USA 77, 6368-6370
A nondenaturing zwitterionic detergent for membrane biochemistry: Design and synthesis.
M.W.: 614.88
Assay (from N): 99.94%
TLC: one spot
Loss on Drying: 0.96%
Solubility (10% in water, 25ºC): Clear and colorless
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent for solubilizing membrane proteins and used for protein solubilization in isoelectric focusing and
especially for non- denaturing (without urea) IEF. CHAPS has been shown to give excellent resolution of some subcellular breaking protein-protein interactions.
Commonly two-dimensional electrophoresis, preparations and plant proteins.
Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT),
Furthermore, its small micellar molecular weight (6,150) and high critical micellar
concentration (6 - 10 mM) allow it to be removed from samples by dialysis.
CHAPS allows the isolation of many proteins in their native state, without loosing much of its activity. Protein-protein interactions can be interrupted more efficiently as with Triton X-100 or sodium cholate.
CHAPS interferes with the Lowry protein assay.
Working Conc.: 1-13mM
Hjelmeland, L.M. (1980) Proc. Natl. Acad. Sci. USA 77, 6368-6370
A nondenaturing zwitterionic detergent for membrane biochemistry: Design and synthesis.
Store at Room Temp.