C17H26N8O5·HCl
M.W.: 458.9
Assay: ≥98%

Blasticidin is an antibiotic commonly used in gene selection. Blasticidin is active against both propkaryotic and eukaryotic. Resistance to blasticidin is conferred by the blasticidin resistence gene from bacillus cereus (bsr), bls or BSD. Bsr codes for blasticidin deaminase.

Blasticidin S is used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or BSD. Blasticidiin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells . It is also used to study protein synthesis at the level of peptide bond formation.

Stock conc.
5-10mg/mL in ddH2O. Sterilize by filtration through a 0.22um filter.
Store at 4℃(Short term) or -20℃(Long term, avoid freeze-thraw cycle)

Working conc.
E.coli: 25-100ug/mL
Yeast: 25-300ug/mL
Mammalian Cells: 1-10ug/mL 
 

Store at 4℃.
Handling: Warning! Very toxic!
Store in Tightly Sealed Vial. Protect from moisture.