C6H16NNa2O12P
Molecular Weight: 371.15 g/mol

    4-Nitrophenyl phosphate disodium salt hexahydrate is a substrate of the hydrolase alkaline phosphatase. The products of the enzymatic reaction are 4-nitrophenol and phosphate.

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
● as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA)
● as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay
● in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample

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