產品櫥窗
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Bicine
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產品型號：101-150-25-4 商品規格： |
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Molecular Biology Buffer. Synonym: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4
M.W.: 163.17
Assay (Titration): 99.46%
pH(1M in water): 4.8
A260 (1M in water): 0.0190
A280 (1M in water): 0.0064
White powder
Bicine is a zwitterionic buffer that is used in biochemistry and molecular biology research. Originally prepared in the 1920's, it later became one of the Good buffers that were studied in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.
The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer.
A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.
Versatile buffer. Its applications last from enzyme reaction buffers (T4 polynucleotide kinase; Micrococcus nuclease; adenylate cyclase ref. 6) to electrophoresis buffers
Interferes with the BCA and Lowry protein assay.
Working Conc.: 3-100mM
M.W.: 163.17
Assay (Titration): 99.46%
pH(1M in water): 4.8
A260 (1M in water): 0.0190
A280 (1M in water): 0.0064
White powder
Bicine is a zwitterionic buffer that is used in biochemistry and molecular biology research. Originally prepared in the 1920's, it later became one of the Good buffers that were studied in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies.
The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer.
A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.
Versatile buffer. Its applications last from enzyme reaction buffers (T4 polynucleotide kinase; Micrococcus nuclease; adenylate cyclase ref. 6) to electrophoresis buffers
Interferes with the BCA and Lowry protein assay.
Working Conc.: 3-100mM
Store at Room Temp.