產品櫥窗
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BES
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產品型號：101-10191-18-1 商品規格： |
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Molecular Biology Buffer. Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
C6H15NO5S
M.W.: 213.25
Assay (Titration): 99.78%
A260 (1M in water): 0.0600
A280 (1M in water): 0.0534
Highly efficient transformation of mammalian cells with a BES-buffered salt solution (50 mM BES, pH 6.95(transformation crucial factor); 280 mM NaCl; 1.5 mM Na2HPO4).
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.
A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.
Chen, C. & Okayama, H. (1987) Mol. Cell. Biol. 7, 2745-2752
High-efficiency transformation of mammalian cells by plasmid DNA.
M.W.: 213.25
Assay (Titration): 99.78%
A260 (1M in water): 0.0600
A280 (1M in water): 0.0534
Highly efficient transformation of mammalian cells with a BES-buffered salt solution (50 mM BES, pH 6.95(transformation crucial factor); 280 mM NaCl; 1.5 mM Na2HPO4).
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.
A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.
Chen, C. & Okayama, H. (1987) Mol. Cell. Biol. 7, 2745-2752
High-efficiency transformation of mammalian cells by plasmid DNA.
Store at Room Temp.