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產品櫥窗

  • Excel Super-Fidelity DNA Polymerase
  • 產品型號:VZ-505P

    商品規格:

    商品售價

High fidelity DNA polymerase, which can amplify long fragment up to 40kb. It is compatible with high GC-content samples and can directly be used in crude extract.

    ExcelTM Super-Fidelity DNA Polymerase is a new generation superior enzyme based on ExcelTM DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to ExcelTM Super-Fidelity greatly improve its long-fragment amplification ability, specificity, and PCR yield. P ExcelTM Super-Fidelity is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of ExcelTM Super-Fidelity is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, ExcelTM Super-Fidelity has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. ExcelTM Super-Fidelity contains two monoclonal antibodies inhibiting the 5’->3’ polymerase activity and 3’->5’ exonuclease activity at room temperature, which enable ExcelTM Super-Fidelity to perform greatly-specific Hot-Start PCRs. The amplification generates blunt-ended products, which need additional A-tailing step for compatible with general TA cloning kit.

Features:
High speed amplification


Amplification of  long fragment up to 40kb

Compatible with high GC-content

Can directly be used in crude extract

Components:
  100 U 500 U 1000 U
Excel Super-Fidelity DNA Polymerase (1 U/µl) 100 µl 5× 100 µl 10× 100 µl
2× Excel Super-Fidelity Buffer 1.25 ml 5× 1.25 ml 10× 1.25 ml
dNTP Mix (10 mM each) 100 µl 5× 100 µl 10× 100 µl
10× Loading buffer 1.25 µl 5× 1.25 µl 10× 1.25 µl

Store at -20℃;
avoid repeated freezing and thawing.