產品櫥窗
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Zeocin Solution, 100mg/mL Soln.
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產品型號：900001 商品規格： |
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Selection antibiotic.
C55H86N20O21S2
M.W.: 1427.53
M.W.: 1427.53
Zeocin is a glycopeptide antibiotic of the bleomycin family. It is active in vivo against most bacteria (including E. coli), eukaryotic microorganisms (i.e. yeasts), plant cells, and animal cells.
Usage: Liquid Zeocin™ is ready for cell culture.
The concentration of Zeocin required for selection of transformants is pH and salt concentration dependent. The higher the pH and the lower the salt concentration, the better is Zeocin activity.
Selection of common E. coli strains is achieved at 25 μg/mL zeocin in Low Salt LB Agar medium adjusted to pH 7.5.
Animal cells: 100 to 750 μg/mL depending on the cell line.
Plant cells: 50 to 250 μg/mL depending on species and media.
Yeasts: Depends on species and media. S. cerevisiae transformants are selected at 50 μg/mL in YEPD at pH 7.0.
The concentration of Zeocin required for selection of transformants is pH and salt concentration dependent. The higher the pH and the lower the salt concentration, the better is Zeocin activity.
Selection of common E. coli strains is achieved at 25 μg/mL zeocin in Low Salt LB Agar medium adjusted to pH 7.5.
Animal cells: 100 to 750 μg/mL depending on the cell line.
Plant cells: 50 to 250 μg/mL depending on species and media.
Yeasts: Depends on species and media. S. cerevisiae transformants are selected at 50 μg/mL in YEPD at pH 7.0.
Upon receipt it should be stored at 4°C or -20°C.