https://www.high-endrolex.com/17
產品櫥窗-塞魯士生技有限公司-最高品質,價格平實。您最好的研究夥伴。

塞魯士有限公司

免付費服務專線:080-007-2222

產品櫥窗

  • TAE Buffer 50X
  • 產品型號:2003-50

    商品規格:

    商品售價

For DNA Electrophoresis.

TAE and TBE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation.

 

50X TAE stock solution (1 Liter) contains:
242 g of Tris base
57.2 mL of glacial acetic acid
100 ml of 0.5 M EDTA (pH 8.0)
Ready to use. Before use, add 49X of ddH2O to dilute.
 

 

Store at Room Temperature.