產品櫥窗
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Stripping Buffer for Western Blot
產品型號:20073 商品規格: |
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A reagent for removal of primary and secondary antibodies from a western blot membrane.
Nitrocellulose and PVDF membranes that have been probed by Western blotting procedures and detected by chemiluminescent or other nonprecipitating substrates may be used to detect tubulin, actin and other stable-expression proteins as a consult or to compare with the other proteins.
Western Blot Stripping Buffer provides a robust but gentle method for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane. Compared with a new SDS-PAGE, it is much simpler and easier and can avoid the error causing by new loading.
Advantages:
Stripping high-affinity antibody;
Effective use of samples that are available in limited amounts;
Multiple times of re-probes (~20 times) ;
Comparison of images obtained with different antibodies in the same blot ;
Confirmation of results with the same or different antibodies ;
It is simply more economical and less time consuming to reuse blots for re-probing and mass spectrometry.
Western Blot Stripping Buffer provides a robust but gentle method for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane. Compared with a new SDS-PAGE, it is much simpler and easier and can avoid the error causing by new loading.
Advantages:
Stripping high-affinity antibody;
Effective use of samples that are available in limited amounts;
Multiple times of re-probes (~20 times) ;
Comparison of images obtained with different antibodies in the same blot ;
Confirmation of results with the same or different antibodies ;
It is simply more economical and less time consuming to reuse blots for re-probing and mass spectrometry.
Store at room temp.
Store at 4℃ for long term storage
Store at 4℃ for long term storage