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  • Fast Green FCF
  • 產品型號:101-2353-45-9

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For protein staining in IEF, native PAGE and SDS-PAGE. Synonym: Food green 3

C37H34N2O10S3Na2
M.W. 808.85
Purity: ≥95%
Appearance: dark Brown to Red-Brown,powder
MP: 290℃(dec.)(lit.)
Solubility in water: 1mg/ml, clear, blue-green to dark blue

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R.
After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R. 
Store at RT, avoid direct sunlight.
Once opened keep powdered medium closed to avoid hydration.